The route leaves Castro Marim, whose castle faces Ayamonte, and heads north following the Guadiana on the EN 122 and local roads. It passes through Azinhal and Odeleite and ends in Alcoutim, a white village on the riverbank opposite Sanlúcar de Guadiana.
This is the inland Algarve, very different from the coast: gentle hills covered in rockrose, stream valleys and small villages, with the river and the Spanish bank appearing now and then. It crosses the municipalities of Castro Marim and Alcoutim.
It is about 41 km and just over an hour without stops, with more than 100 gentle bends. Altitude ranges from almost river level to about 220 m. It is a quiet ride with little traffic, made for enjoying the scenery.